FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300560
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA27340.21021713723319185No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC24710.18999507904287383No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA21740.16715876237928276No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT21640.1663898628283201No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC20000.15377991019253243No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT19950.1533954604170511No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC18930.14555268499723198No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG15650.12033277972565665No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT13320.10241742018822662No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG8900.040.7284439
GTCGCGC9300.039.7562188
TCCCCCC6600.039.5436178
CCCCCCG6700.038.9534159
TATAGGT8850.038.506963
GGTCGCG9500.037.3937
AGGTCGC10150.035.718126
CCGATCT14700.034.034274
ACTCCCC9250.033.7063266
TTCCGAT15500.032.744192
CTAGGGG1601.8761166E-631.7221583
CTTCCGA16500.030.7596971
CTCCCCC9550.028.0877027
ACCATAC9850.027.235381
CCATACT10650.026.5511252
GTACAGG2259.10557E-725.7795561
TAAGGTG9900.025.6340665
TAGGACA5150.025.3425274
TACTCCC12300.024.7587575
CCTATAA15600.024.6331081
Produced by FastQC (version 0.11.2)