FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356216
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGGA400.00605038854.3688964
AGTACCC604.9400807E-448.334695
CCTTAGG604.943503E-448.3279082
CTTAGGG604.943503E-448.3279083
ATAGGAT803.7322563E-545.307413
GTATAGA700.001056307741.423921
TAGACCA1406.592254E-736.251025
TACTCCT1001.39057E-436.2459264
GTATAGG1001.39057E-436.2459261
CAGTACC1051.8525496E-434.519934
TCTTAGG850.002741136634.113812
CATAGAT1501.1321663E-633.8295332
TCCAGTA900.00362711432.2186052
CTGTGCA1153.1575238E-431.5226239
CTACGCT1153.1601253E-431.5181984
ACTGTCC1402.7412276E-531.0723028
TAGATCG1652.380215E-630.758449
GGATAGT950.004722605530.527176
GAGACAG950.004722605530.527175
TAGACCC950.0047258530.5228884
Produced by FastQC (version 0.11.2)