FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568097
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG12490.21985682022612335No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG8960.15771954437358407No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8120.14293333708856057No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC7370.12973136629836102No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6010.10579179259879916No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACA450.00963393548.3372342
TACAGAG1451.9239451E-840.003232
ACGTTAC750.001482257738.6697921
CTATACG1701.8244464E-938.3854523
TCTATAC951.0278409E-438.1609763
CTAGACG1001.3900324E-436.2529263
CTATACT1001.3900324E-436.2529264
CTTCCGA20100.035.3511121
TTCCGAT20450.035.1006362
ATACCCG5400.034.9102255
CCGATCT20800.034.514
TATAGGT4850.034.384223
TAACGTT7100.033.69994
ACCTATA5150.032.3812561
CCATAAG900.003625487732.2248232
TCCGATC22800.032.118823
TATAAGC2807.2759576E-1231.0739363
CTAACGT7600.030.5287823
TATACGT1902.0724474E-730.5287824
AACGTTG7900.030.2872565
Produced by FastQC (version 0.11.2)