FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531498
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA10230.19247485409164286No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10040.18890005230499457No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA9380.1764823197829531No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8920.16782753651001509No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8510.16011349054935298No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8300.15616239383779432No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7790.14656687325258044No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG6350.1194736386590354No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5850.11006626553627671No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT5630.10592702136226288No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5400.1015996297257939No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCC2900.037.495678
CCCCCCG2950.036.860159
CTTCCGA5550.032.6631051
TTCCGAT6200.031.5748752
GTCGCGC3950.031.1989278
TCGCGCG3600.030.2048449
CTCCCCC3650.029.791087
GTATAAT2202.2562745E-829.664041
TATAGGT4300.028.6648733
CCGATCT6500.027.8866424
CTAACGT3403.6379788E-1227.7226033
GGTCGCG4350.026.6635887
TAGACAG4650.026.5073035
CTAGTGC1100.0096641626.3655513
GGGTGCC1100.0096730326.360597
CCGCGCA1100.0096730326.360599
AGGTCGC4550.025.4963596
GTCTAAC2351.326549E-624.6849691
TCTATAC2351.3276367E-624.6826443
GTGTAGG2651.3715726E-724.6267491
Produced by FastQC (version 0.11.2)