FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531742
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG15040.28284393559282506No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG14480.2723125124590497No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC13460.25313027746538735No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC9590.18035062116590375No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC7630.14349064019768984No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT6540.12299197731230559No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC6500.12223973280275022No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC6360.11960687701930636No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT5950.11189637079636365No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT5860.11020382064986403No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA5840.10982769839508633No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT5720.10757096486642018No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA5560.10456198682819864No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5560.10456198682819864No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG5460.10268137555431017No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA5360.10080076428042171No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG5350.10061270315303286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA700.001054247341.4441076
TATAGAC1053.815827E-641.4363142
ACTAATG907.47362E-540.2815138
CTAACGT7750.036.4975553
CTAATGG800.002034832336.253369
ATACCCG8900.034.2263155
TAGGGTG850.002735801534.130445
GTATACG1501.130913E-633.8396531
TTCCGAT17700.033.5938452
CTTCCGA18150.033.5599861
CCGATCT18250.032.9849664
ATAGGGT1551.4590169E-632.7542154
ACCTATA9100.032.670941
AACGTTG9000.032.2343065
CTTCTAG1352.137593E-532.228241
CTAGGAC6350.031.9804923
TAACGTT8850.031.9611364
TACCCGG9950.030.6144926
ACGTAGT950.004721008230.532022
GTATAGA1204.0510777E-430.2139781
Produced by FastQC (version 0.11.2)