FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5f11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5f11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613330
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC1157.124103E-637.836981
TCTATAC1752.421075E-937.293413
TATAGGT2800.036.257483
ATACGAC1251.2625047E-534.8071823
CTATACT1102.4321074E-432.9613464
TATACCG2151.8044375E-830.3551035
GTGTTAA1255.1428674E-429.0083521
GTACTGA1804.6855585E-628.2025641
CCTAGTG1306.472251E-427.8903712
CTTCCGA17700.027.8611281
TTCCGAT18100.027.243192
TAAGGGT1606.81432E-527.1931114
GATCTTT5750.026.4837256
TAAGTCA1100.00965900326.3690764
TCCGATC19400.026.1651943
TATACTA1409.978145E-425.8982015
AATACCG2559.454743E-825.5935175
ATAGGTC4450.024.4432474
GTACTAC1500.001491112824.1736261
CCGATCT22000.024.0617854
Produced by FastQC (version 0.11.2)