FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5f10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5f10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746847
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG21350.28586845766268054No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG14400.19281057566007495No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC11400.15264170573089267No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC11230.150365469768239No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA8410.11260673203480767No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC8130.10885763750808398No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGG502.3020639E-672.51912
GTCTAGG700.001054406241.445031
TCTAGGG800.002032986836.2619743
TTACACT1458.6720684E-735.0115624
GGCCCTA1801.2924647E-732.2199177
CCGATCT35950.030.6638134
CTTCCGA35050.030.625571
TTCCGAT35350.030.3615992
CTATACT1204.0492273E-430.2183114
TATAGGT6400.029.4628523
TCCGATC37800.028.7793453
ATACCCG7350.028.6148915
CTAACGT12350.027.6002063
TAGACGT2903.929017E-1027.5090834
CTAGACG2156.103455E-726.9856553
TATTAAG2156.1070386E-726.9838452
TAACGTT12700.026.8395714
GTATTAA3800.026.7211381
TAGGGAA1907.133929E-626.7193495
CCTAACG13850.026.703782
Produced by FastQC (version 0.11.2)