FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169701
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT38650.033.5753362
CTTCCGA39150.033.3331341
TCCGATC41300.031.5978763
CCGATCT41100.030.8696464
TAGACGT3850.028.2465864
TAGGACA2903.965397E-1027.4998364
GTATAAG5350.027.1026421
CTGTCTA5100.027.020042145
TAGACTG2001.0657188E-525.3748515
CCGTATA2701.6501508E-724.1654912
CGATCTT18050.023.697925
TCTAGGA2451.9133531E-623.672322
TATAAGG5750.022.6945482
GATCTTT15500.021.983746
CGTATAC4358.367351E-1121.6665383
GTACAGA3355.5011697E-821.6416651
CGATCTG20950.021.455725
TATAAGA5750.021.4337392
ACCATAC5251.8189894E-1220.7141631
ACACATA9950.020.409742145
Produced by FastQC (version 0.11.2)