FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236107
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC27880.22554681754896624No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA25790.20863889614734No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA22580.1826702704539332No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT22510.18210397643569692No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC22170.1793534054899778No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT21920.17733092685341964No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC20500.16584324819776927No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG18200.1472364447414342No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT16930.13696225326771874No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT15430.12482738144836976No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG14300.11568577801112687No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT13700.11083182928338729No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT13590.1099419386833017No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA12500.1011239318279081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT13050.040.0211144
TCGCGCG10200.038.3808679
GTCGCGC10250.038.193648
TTCCGAT14300.036.016962
TATAGGT11350.035.1507033
CTTCCGA14450.035.142491
GGTCGCG11750.033.317867
AGGTCGC11650.033.0015756
TCCCCCC8200.031.8280338
CGATCTG8250.031.6530655
CCTACAC8700.029.1820623
CTAGGGG1804.6818805E-628.2093263
CCCCCCG9350.027.9133579
CCTATAA16300.027.5934751
ATACCCG11850.027.5461795
CCATACT9950.026.975122
CTATAAG17100.026.3014892
CTACACC9950.026.2450034
TACCCGG12300.025.94976
CGCCTAG4200.025.908621
Produced by FastQC (version 0.11.2)