FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864028
Sequences flagged as poor quality0
Sequence length151
%GC61

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG750.001480692238.6803781
TACTATA850.00273779534.127772
GCTAATA900.003622692832.231782
TAGACAG1902.0706466E-730.5353745
GTCTAGA1204.050548E-430.2172952
TTTAAGG1703.0041356E-629.8617973
ATTCTAA1306.468804E-427.8945031
TCCTAGA1050.00769672827.6272412
CTAGCGT1050.00769672827.6272414
CCCTAGC1606.813306E-527.1955642
ATACTAT1358.067825E-426.8598186
GTGTAGG3306.184564E-1126.3729841
CAGTACT1658.4003E-526.3714584
CTAACGT1100.00965689626.3714563
CTATTAA1409.972834E-425.9020371
TATAATG2001.0628019E-525.3825262
CCCCTAG1450.001223775825.0088651
GGTATAC1450.001223775825.0088651
TAAGGTG1751.2530059E-424.8645175
GTCCAAT2101.5504864E-524.1752381
Produced by FastQC (version 0.11.2)