FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481770
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA28250.19065037084027886No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC27130.1830918428636023No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA24020.16210343035693797No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT23810.16068620636131115No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT20330.13720078014806616No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA19300.13024963388380112No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC18740.12647036989546287No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT15560.10500954939025625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTACC6000.039.8837145
TATAGGT8800.038.7261163
TCGCGCG8600.037.9327969
AGGTCGC9900.035.1556246
TCCCCCC8950.034.8294228
GGTCGCG10150.032.854337
GTCGCGC10050.032.4599088
CCCCCCG10050.031.0172449
GTCTACG10900.029.2714521
ACCATAC12650.028.0881821
TACGAGT12000.027.7949144
ATACTCC12400.027.483054
CTCCCCC11400.027.344157
TAGGACA8000.027.1906764
CCATACT13300.026.168472
CATACTC13850.025.6528173
AGGTGCC13600.025.5860447
TAAGGTG14000.025.3779645
CCTATAA20350.025.2995051
TAGGTCG14050.025.2876515