FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835101
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA19030.22787662809648176No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC18360.21985364644516053No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT16080.19255155963170922No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15880.19015663973579242No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC14220.17027880459968314No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT14210.1701590586048873No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC13780.16500998082866625No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG10450.1251345645616518No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT9380.11232174311849705No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT9370.1122019971237012No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA9300.11136377516013034No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT9140.1094478392433969No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT9090.1088491092694177No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG9090.1088491092694177No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA8560.10250257154523824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT9600.037.7679522
CTTCCGA9800.036.2572331
TCCCCCC5600.033.661388
TATAGGT6150.033.0166933
TATACTG2950.031.9574385
CCGATCT11050.031.5013834
GTCGCGC6250.031.320628
TCGCGCG6150.030.6510149
GGTCGCG7150.029.4061787
CGATCTG6050.028.767795
CCCCCCG6600.028.561179
CCTATAA11750.028.3886411
ATACTCC6650.028.3532184
AGGTCGC7450.028.2287966
ACCATAC7050.026.7429241
TACTCCC8700.026.6735845
CATACTC7350.026.639563
CTATAAG12800.026.0598832
CCATACT7800.026.0308322
ATACCCG6750.025.7844645
Produced by FastQC (version 0.11.2)