FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333103
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.2515738375217275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6540.19633566794655105No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6120.18372695532613037No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.15490704076516873No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.12338525921411696No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.1044721902834859No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCA400.006042800454.385362
GCTAGTG400.006042800454.385362
ATTAGGC752.5440208E-548.3425453
ACACACG1551.4551915E-1146.7831081
CCATAGC657.3226326E-444.6238861
TAGGGTA657.3226326E-444.6238864
TAGGCAG907.46329E-540.2854545
GATCTAG1157.119066E-637.8332941
CTGTACT1209.532248E-636.256914
TCTATAC1406.5821587E-736.256913
TACACTG1251.260914E-534.806635
TAGGGGA850.00273681534.124154
GATCTTT8400.032.8038676
TAGACGT2257.876224E-1032.2283634
CTATACT900.003621402732.2283634
TAATGTG900.003621402732.2283635
TACACTA900.003626732932.218697
CTTCCGA17150.031.2887611
CCTGTAC1652.3765897E-630.7634353
AGGCAGT950.004718417330.5321336