FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520679
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5930.11388974781007108No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG803.7268015E-545.3235441
GTCATAA2151.2732926E-1137.1020661
ACACATA4550.036.653767145
TCTAGAC1001.3885403E-436.258842
ATGTACG800.002033285636.2588355
GATCTAG800.002033285636.2588356
ACCTATA3050.033.28681
TTCCGAT18450.030.6578792
CTTCCGA18250.030.5964981
TACTCGG950.004719604330.5337585
TGTACCG2151.8026185E-830.3562375
CTAGACA1204.0496117E-430.2156964
ATACCCG3201.8189894E-1229.4603045
CTAGACG1255.1431445E-429.0070693
CAGTAGT1306.469513E-427.8914154
CCTTACA1050.007695952427.625782
TCCGATC20500.027.2383483
CCGATCT20650.027.0404874
TAGACGT2456.410846E-826.6391474
TAAGGTG2756.73208E-926.3700645
Produced by FastQC (version 0.11.2)