FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622366
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC29510.18189483753974134No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA28080.17308055025807986No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT25180.1552054222043608No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA24000.1479320942376751No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT22410.13813159299442912No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC22140.13646735693425527No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG20700.12759143127999478No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC20110.12395476729665192No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA19900.12266036147207227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCG12900.033.163695
TATAGGT11250.032.226863
CTTCCGA22350.031.4737341
TTCCGAT23250.030.8763622
TACCCGG13450.030.7302766
ACCTATA13400.030.3066061
CCGATCT23600.030.1102664
GGTCGCG14000.027.4427647
CCTATAA18800.027.3876381
ACACATA12300.027.11856145
CTATAAG18350.026.4760172
TCGCGCG13750.026.3601179
AGGTCGC14100.026.2279876
TCCCCCC8850.026.211198
TATAAGC20200.025.4863413
GTCGCGC15250.025.193368
CCATACT10450.023.592642
TAAGGTG14050.023.2239845
ATGTAGG9300.022.613571
CCCCCCG10050.022.3601999
Produced by FastQC (version 0.11.2)