FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5e12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5e12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162774
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA26850.028.0864351
GTATAAG1555.495179E-528.0690121
GTATATG2603.7143764E-927.8890821
ATAAGAC1606.819637E-527.1930243
TTCCGAT26800.027.0554072
TAGGTCG8050.026.123325
TATAGTG2507.817653E-826.1053055
CTGTCTA5200.025.100174145
ACCTATA7350.024.6638151
GTATAGA2651.3745921E-724.6265851
TATAGGT6850.024.3480133
CTAACGT3902.0008883E-1124.171583
TCCGATC30300.023.9322573
CCCTAAC4403.6379788E-1223.0718781
TAGGGGT2852.774159E-722.899394
TATACTG4154.3655746E-1122.7154585
ATACCCG8450.022.3122255
CCTATAG1650.00259804521.9741613
CTAGGAC3406.419941E-821.3278643
CCGATCT35250.021.1887024
Produced by FastQC (version 0.11.2)