FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5e11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5e11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899308
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC20530.22828663817068234No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA19770.21983569589061813No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT17050.18959021825670402No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT16520.18369679798244873No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA16240.18058329293189876No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC16030.17824816414398625No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC14750.16401499819861493No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT11480.1276537070725491No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT11150.12398421897725807No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG10990.12220507323408665No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG10650.11842438852984741No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT10410.11575566991509026No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT10390.11553327669719385No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA9860.10963985642293851No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC9050.10063293109813323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA12650.038.9880751
CCGATCT12650.038.410454
TTCCGAT14150.036.3887372
TCGCGCG6450.033.717679
ATACTCC7150.033.4713334
GGTCGCG7000.033.1396527
TCCCCCC6350.033.1070378
GTCGCGC7100.032.6728978
AGGTCGC8150.031.144086
CCCCCCG6550.030.9893729
TATAGGT7400.030.3805123
ACCATAC7700.029.2001021
CCATACT8550.027.1424432
ATACCCG6300.026.4760025
GTCTAGG2207.4582204E-726.3742871
CTCCCCC8000.026.2787117
CATACTC9550.025.819073
CCCCTAG1450.001223463125.01011
ACCTATA7250.025.01011
ACTCCCC8500.024.7425356
Produced by FastQC (version 0.11.2)