FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5e10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5e10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743541
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15250.20509965153232979No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA14650.19703015704581184No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA12640.16999735051597692No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12340.165962603272718No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT11980.16112090658080724No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC11710.1574896340618742No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC10470.14081267878973722No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT8010.10772775139501385No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG7650.10288605470310314No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT7620.10248257997877723No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT7620.10248257997877723No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG7590.10207910525445132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC4450.047.2428328
TCGCGCG4500.046.7179079
GGTCGCG4650.045.2108847
TATAGGT5250.041.444263
AGGTCGC5550.039.204036
CCTACAT2350.037.0352973
CTTCCGA9650.033.8233681
CCTATAA9350.032.5813671
CCGATCT10100.031.5961194
TTCCGAT10700.029.8243732
CTATAAG10700.029.1465472
TCCCCCC4550.028.6786698
TGCCTAC2553.0067895E-928.4440521
TATAAGC11200.027.8453623
CCCCCCG4700.027.7633959
TAGGTCG8200.026.5344335
TAGGCGG1658.3940715E-526.373624
CTCCCCC5300.025.9881677
ATAGGTC9050.025.6450654
CCATACT5700.025.4482272
Produced by FastQC (version 0.11.2)