FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614671
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG17340.2821021326856155No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG14960.24338223212092325No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC10930.17781870301348202No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC9380.15260196104908155No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8730.14202719828981683No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT7290.11860003156159962No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC7130.11599701303624216No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA7090.1153462584049028No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6850.11144173061686659No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT6610.10753720282883038No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG6180.1005415905419322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGT6850.040.2307173
CTAGGGT750.001480815438.677954
TTCCGAT17000.038.390432
CTTCCGA18000.036.2576261
TTAGTAC1051.8497118E-434.5338863
TCTAACG900.00362197132.2316253
CCGATCT20050.032.1914374
GTGTTTA1402.7455982E-531.0703839
TCCGATC21200.030.7872853
ACCTATA9250.030.5741
CGATCTA4100.030.0697485
TAGTACA1453.4812867E-530.0087554
TATACTC1000.006064007529.0084655
TTACACG1255.142765E-429.0084634
TATAGGA2003.2449134E-729.0061042
CTAACGT11350.028.752883
AGTACAG2054.0245868E-728.300945
CCTAACG11950.027.9138242
TAACGTT11800.027.6563744
TTAGTGC1050.0076952127.6271083
Produced by FastQC (version 0.11.2)