FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124410
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3760.30222650912306087No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC2830.22747367574953783No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG2440.1961257133670927No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC1990.15995498754119444No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.12458805562253839No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA1540.1237842617152962No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1520.12217667390081184No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT1490.11976529217908528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.11574632264287436No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1430.11494252873563218No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG1350.1085121774776947No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA1310.10529700184872598No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA1260.10127803231251507No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACTA350.00355428262.1644025
TACTACT350.00355428262.1644024
CTTCCGA3650.055.6133541
TAGACGT805.8178375E-754.393854
TCTTATC400.00603020954.393858
TAGGGAT400.00603020954.393854
TAGCTGC400.00603020954.393856
ATTCTTA400.00603020954.393856
TTTAGGG553.208918E-452.724352
TTCCGAT3900.052.04842
CTAAGGT450.0096062948.3500864
GATTACG604.9296365E-448.3306581
GTTTAGG450.00962156748.3306541
TCCGATC4250.047.781263
CCGATCT4450.045.6337934
TATAGGT1053.78806E-641.4429323
TACAGGA700.001053361141.4262772
GATAATC750.001475875438.680073
TTAGAGT750.001475875438.680074
AGGAATA750.001475875438.680075
Produced by FastQC (version 0.11.2)