FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232482
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.1410861916191361No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.10108309460517373No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGC451.1914561E-464.449711
CACTATA450.00962679248.337281
TTAGGGT657.31421E-444.628633
CATAACA1105.243919E-639.548682
GTCTAAA750.001480447238.6698231
CTTCCGA11100.035.2731481
GGACAGA1051.8461261E-434.534066
ACCATAC1051.8484636E-434.526631
TTCCGAT11450.034.1949272
AACACTG850.00273369434.1277735
TCCGATC11600.033.7600173
TCCTACT1301.648421E-533.464272
CCGATCT11750.033.329044
CCATACT900.003621097432.2248542
ATTGGGA950.004713059430.535383
AATGGTC1204.041523E-430.21735
TAGCGCG1753.7369937E-629.021101145
CCTAAGA1255.132892E-429.008613
AGGCAGT1504.3754982E-529.008616
TGGGATG1255.132892E-429.008615
Produced by FastQC (version 0.11.2)