FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306966
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6770.22054559788380473No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6630.2159848321963996No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6180.20132522820116883No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5680.18503677931757914No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5370.17493794100975352No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5350.17428640305440993No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT5230.1703771753223484No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT4360.14203527426490228No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4160.13551989471146642No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG4070.13258797391242028No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT3780.12314067355993824No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3760.12248913560459465No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3580.11662529400650234No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA3110.101314152055928No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGG350.003565022762.1390157
TAGGCGG502.0054198E-458.015324
TATACAG1153.8198777E-1156.7541125
ATACAGT1203.597961E-948.34616
TATAGGT2600.047.4163633
GTATTGA1402.7102942E-1046.619451
GTCGCGC2500.046.3971338
GGTCGCG2500.046.3971337
TCCCCCC2300.044.127718
CCCCCCG2350.043.188829
TCGCGCG2700.042.9603089
TCTAGAC700.001054125341.439513
ACCATAC2300.040.9890821
CTAGACA907.4586875E-540.2884144
ATACTCC2700.040.2884144
AGGTCGC2900.040.0105636
CCATACT2850.038.1679732
CTAGCAC951.0256302E-438.167973
CTTCCGA3700.037.239561
GCTAGGG800.002031784536.259572
Produced by FastQC (version 0.11.2)