FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082836
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC16870.1557945986280471No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16380.15126944431104988No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14140.13058302457620544No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC12820.11839281294674356No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT12370.11423705898215428No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11910.109988954929463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCC6350.035.3918088
CCCCCCG6750.032.22059
CCGATCT13800.030.4750144
CTTCCGA13800.030.4721981
CTCCCCC8000.028.998457
TTCCGAT15150.028.2354222
CCATACT9000.027.3899522
TCGCGCG6300.026.4668399
GGTCGCG7200.025.1722647
TACTCCC11200.024.6014425
TATTAGG2951.4275429E-824.5772272
TATAGGT7100.024.5102583
CATACTC10950.024.5009333
ACCATAC10450.024.283241
AGGTCGC7250.024.003156
TATACCG2151.8640274E-523.6077485
GTCGCGC7100.023.4846578
TAAGGTG11550.023.2281575
ACTCCCC10100.022.9733126
AGGTGCC11400.022.257587
Produced by FastQC (version 0.11.2)