FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012418
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC16870.16663077898654508No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16590.16386512290378086No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14780.14598713179734063No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC13350.13186253108893758No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT13250.13087479677366462No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12610.12455329715591781No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA10210.10084767358936723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA10750.046.5454441
TTCCGAT12300.041.8570252
CCGATCT12300.041.2695274
GTCGCGC5700.036.881588
TCGCGCG6200.033.907269
GGTCGCG6300.033.369057
AGGTCGC7100.031.6621136
TATAGGT7300.030.7946593
GTCTAGG1606.817677E-527.193671
TAAGGTG9100.027.0940575
TCCCCCC4750.025.9442848
GGGCGAC2351.331844E-624.6779177
AGGTGCC8400.024.1637957
CCCCCCG5400.024.1637949
CCTATAA13350.023.3573591
TGTACTG4053.274181E-1123.2768865
CGATCTA5000.023.2052655
GACCGTG3753.074092E-1023.1972437
ATAAGGT10050.023.0898154
CGATCTG8200.022.993025
Produced by FastQC (version 0.11.2)