FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001354
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC19410.19383754396547076No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA17660.17636120692582244No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15470.15449081943049112No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC14790.1477000141807992No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT13880.13861231892018208No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT13720.1370144823908428No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC13160.13142205453815534No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT11420.11404558228159073No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT10670.10655572355031287No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG10350.10336005049163433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA12950.041.996591
CCGATCT12800.041.9222224
TTCCGAT13150.041.9092942
GTCGCGC6100.040.4055948
TCGCGCG6300.039.1228759
GGTCGCG6750.037.5886467
TATAGGT6650.037.0748833
CGATCTG7700.034.844445
AGGTCGC7250.034.0066156
CCTATAA12800.032.8579561
CTATAAG13150.030.8805332
TATAAGC13650.028.6869013
GTCTACG8850.028.6778981
CGAGTCG7650.028.4369076
TCCCCCC5150.028.1523828
TACGAGT8550.027.1397844
ACCTATA9100.027.0931851
TAAGCAA16200.026.8570795
CGAGGGG1658.4103034E-526.367449145
ATACCCG9850.025.7664345
Produced by FastQC (version 0.11.2)