FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015726
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC18380.1809543124819095No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA18320.1803636019950262No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15950.1570305377631369No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC14270.14049064413040524No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC14140.13921077140882482No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT14120.13901386791319706No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT13940.13724173645254725No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG11090.10918298832559176No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT10950.10780466385619744No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT10410.10248826947424798No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT10300.1014053002482953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG8800.034.5991259
TATAGGT8900.033.4057043
GTCGCGC9350.033.3392148
CTTCCGA9550.031.8944761
AGGTCGC9800.030.3378336
GGTCGCG10100.030.145777
TTCCGAT11300.028.2358592
CCTATAA12650.026.9449211
CCGATCT12100.026.3690284
TAGGGGG1100.00966202226.369026145
TATACAG3657.2759576E-1225.8271965
CTATAAG13500.025.7830472
TACGAGT9100.025.4997184
CCTACAT5350.024.3975123
GTCCTAA5550.023.5206381
CGAGTCG7750.023.3918786
GTCTATA2502.273282E-623.207031
GTATATG3452.9540388E-923.1229461
TATAAGC15650.022.704323
TGCCTAC4850.022.4294741
Produced by FastQC (version 0.11.2)