FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5d11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5d11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739095
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC12750.17250827024942667No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA11820.1599253140665273No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10740.14531284882187No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9960.1347594017007286No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9800.13259459203485344No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT9660.13070038357721267No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8820.1193351328313681No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT7610.10296375973318721No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT7430.10052834885907766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT10050.038.9539534
TTCCGAT9750.037.9192732
TCCCCCC3700.037.2285168
CTTCCGA10900.034.5836871
GGGCGAC2206.220944E-1032.953477
TATAGGT5550.028.7377993
CCCCCCG4800.028.696989
TACTCCC6650.027.2547475
AGGGGTC1606.827282E-527.1866115
AGGTCGC6200.026.8942836
GTCGCGC6250.026.679138
CCATACT5850.026.023032
GTACTAT1701.031165E-425.5873994
CTATTAG1701.0316393E-425.5856671
ACTCCCC6050.025.1644676
CTATACT1751.2555708E-424.856334
CTCCCCC5900.024.5754687
TCGCGCG6200.024.5556499
GGTCGCG6550.024.3503487
ATACTCC6550.024.3503484
Produced by FastQC (version 0.11.2)