FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425670
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG12780.3002325745295652No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG10960.25747644889233445No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8100.19028825146240044No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC7980.18746916625555007No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC5520.12967791951511734No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA5390.12662391054102945No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG4690.11017924683440222No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC4380.10289661005003876No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGG855.3391355E-542.6432273
GGCCCTA1001.3908428E-436.246747
TTCCGAT18450.034.5768362
CTTCCGA18800.034.318721
CGATCTA4450.034.2104075
CCGATCT18650.034.206044
GTCTAAA1501.1326574E-633.830291
ATCTAAC900.003627505932.2193227
TCCGATC20050.031.8175893
GACCATA950.004726357830.5235712
GATCTAA1902.0734842E-730.5235716
ACTGAGG1703.0084684E-629.8502583
GCATACA1000.006073259328.997392
CTAACGT8300.028.822713
CCCTAAC8650.028.4945491
GTGTACA1306.480192E-427.8821091
TACACGG1306.480192E-427.8821095
ATAAGAC1050.007706940627.6165643
CCTAACG8800.027.1850572
CTATAGT1358.0792914E-426.8494364
Produced by FastQC (version 0.11.2)