FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336902
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG8520.2528925325465565No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6610.19619948827849049No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG6350.18848211052472233No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC5340.158503066173546No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5210.15464437729666192No Hit
GTGATAAAGGAGGTAGGGGTGCAGAGACAGCCAGGAGGTTTGCCTAGAAG5020.1490047550919852No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC4960.14722382176419255No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT4710.13980326623172318No Hit
GTACAGGAATATTCACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATC4700.1395064440104244No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4520.13416364402704645No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4510.13386682180574766No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4060.12050982184730277No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4020.11932253296210768No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC3920.11635431074911992No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA3740.11101151076574198No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGATCGAGCGCTCTTGCGCCGAA3580.10626235522496155No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.10329413301197381No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAG1200.066.450176
ATCTTAC350.003566186262.1352237
CTTCCGA12700.059.9424821
TTCCGAT12850.059.242762
CGATCTA2450.059.1764035
CCGATCT13650.054.7002454
ATCTAGC901.3220579E-648.3273967
TCCGATC16800.045.7384263
ATCTGTG1002.731027E-643.494667
ATATACC700.001056280841.423483
ACCTATA4750.041.211581
GATCTTT4100.040.6657376
ATACCCG4950.039.5405965
TACCCGG5050.038.7576186
CGATCTT5800.038.745245
ACAGTCT951.0281831E-438.153218
CAATAAC1157.1346385E-637.821443
TACAGGA2750.036.9100342
GTACAGG2800.036.2509271
TTAGTAC800.002035929636.2455443
Produced by FastQC (version 0.11.2)