FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204399
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12240.5988287613931575No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12100.5919794128151311No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.43346591715223654No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7680.37573569342315766No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.11105729480085519No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG554.368303E-879.1133353
TCTAGAC651.3897625E-766.942062
AGTACTA400.00603724554.3904235
GTACAAT553.2084365E-452.7422261
GTACTAT553.2084365E-452.7422266
TTCTAGA604.9288856E-448.347041
TAGACGT1401.4015313E-841.4403234
ACACACG1001.385264E-436.260281
ATTGGAG800.002030002236.260283
AGTAATG1209.50814E-636.260282
TAACTGG800.002030002236.260286
TTCCGAT11200.035.6127782
CTTCCGA11300.034.655841
CAGACGT1051.845491E-434.53364
GCAGTCC850.002733101634.1273236
GTCTAGA850.002733101634.1273232
CCGATCT11600.033.759574
GATCTTT6150.033.017496
GAGTAAT1102.4253612E-432.963891
CCTACAC1102.4253612E-432.963893
Produced by FastQC (version 0.11.2)