FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140189
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7530.5371320146373825No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.5071724600360941No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.4408334462760987No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.3488148142864276No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.12554480023396986No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT851.6734703E-1068.257474
TCTAGAC502.0010408E-458.018842
CTAATGG502.0010408E-458.018843
AATCGGA902.0272637E-856.407211
TGTAGAC805.8240585E-754.3926662
AGACAGA450.00960963648.3490376
GTGTGTC604.9312215E-448.3317839
CTAGACG657.303222E-444.629883
CGATCTG2650.041.0510645
TTCTAGA750.001476672638.679231
AGACCGG800.002027418536.2617766
CGATCTT5900.033.1887445
CTTCCGA11050.032.8160861
TCCGATC10850.032.752573
CCGATCT10950.032.453464
TTCCGAT11050.032.1597632
GTATATA1402.7239408E-531.0815221
TAATGGC950.004706097330.536234
ACCGACC950.004714323730.5253378
TCTTCCG9050.029.6505155
Produced by FastQC (version 0.11.2)