FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863840
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC17110.1980690868679385No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16700.1933228375625116No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14250.16496110390813112No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC12970.1501435451009446No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11410.13208464530468605No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT10620.12293943322837562No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT10600.12270790887201333No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC10330.11958233006112243No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT10110.11703556214113725No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG9600.11113169105389886No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT9210.10661696610483422No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT8870.10268105204667533No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG5500.040.8592159
GTCGCGC5850.039.653838
TATAGGT6100.036.850943
GGTCGCG6550.035.4160167
CCCCCCG5750.034.0397959
CTTCCGA9200.033.895941
TCCCCCC5850.033.457928
CTAACGT5550.032.6635783
CCGATCT8900.032.5901764
AGGTCGC6900.032.578376
TTCCGAT9600.032.4836042
ACCATAC7250.029.0086151
GGGCGAC1804.702946E-628.1913957
CCTATAA12550.027.7373241
ATACTCC7750.027.1339514
CTCCCCC7250.026.9970787
GTCTAGA2705.5442797E-926.859831
CCCTAAC6950.026.0868851
TACGAGT7700.025.4266854
CCTAACG8600.025.2982122
Produced by FastQC (version 0.11.2)