FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144669
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC19980.17454827552768531No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA19120.1670351865910582No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA16470.14388438928633518No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC15850.13846797633202262No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT14470.12641208943371401No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT13990.12221873746908496No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC12350.1078914515899356No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT11770.10282448463267548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA12500.055.694731
CCGATCT14700.046.368764
TTCCGAT15500.044.9131432
GGGCCGT1153.1590986E-431.5273276
CGATCTG9450.029.9259385
TCCGATC23900.028.82313
CCTACAC6850.028.5817073
GTCTACG9350.025.5950051
TCCTACA8500.025.5938872
CCTATAA14750.025.5660841
TCCCCCC6100.024.9568968
CTATAAG16600.024.9000782
TATAAGC17800.024.8499093
GTACTAT2101.55214E-524.1730611
CGATCTA3601.8371793E-1024.170955
CGAGTCG8100.024.1709486
ATACCCG7550.024.0108785
ACCTATA7650.023.699081
TCTTCCG9850.023.5574725
TCGCGCG5700.022.8927949
Produced by FastQC (version 0.11.2)