FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303124
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.36354759108483653No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8280.2731555403069371No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5610.18507277549781606No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5350.1764954276137818No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4440.14647471001966192No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4210.138887056122247No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT4150.13690766814900832No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.1355880761668492No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3780.1247014423140365No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3710.12239215634525803No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3510.11579419643446245No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3440.11348491046568401No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.10952613451920667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC1051.6370905E-1162.166772
CAGACGT651.0882648E-555.7999044
TAGGACA1051.0986696E-955.268484
ACTGTGC400.006049473354.3690038
TAGACGT1750.049.7416274
TTAGGAC1203.588866E-948.3599133
CTAGACG1307.2850526E-944.6399233
GCATAGA657.31458E-444.6325531
ACAGTAC700.001052622341.4513553
TCTACAC700.001052622341.4513553
TATAGAG700.001052622341.4513555
ACAGATC1105.256139E-639.5410928
AGGACAG750.001477754238.687935
TAACACA1001.384675E-436.2699364
GTATAGC800.0020305436.263951
ACAGATA1406.5950735E-736.2460028
ATACAGT1051.8447041E-434.5427976
CCTGTGC1051.8518772E-434.528
TATACTA850.002731609234.136415
TTCTAGA2353.274181E-1133.9492261
Produced by FastQC (version 0.11.2)