FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025792
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC18760.18288307961068132No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16390.1597789805340654No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14650.14281647741452458No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC13960.13608996755677566No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12960.1263414025455453No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT12620.12302689044172699No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT10640.10372473171949088No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC10640.10372473171949088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT12450.044.2631874
CTTCCGA12450.043.680781
TTCCGAT13200.041.1989172
TCCCCCC6450.037.0909548
TCGCGCG4650.035.8582159
CCCCCCG6750.035.4424679
CGATCTG8100.034.0170825
CTCCCCC8250.028.9983837
GTCGCGC5650.028.2285148
GGTCGCG5650.028.2285147
TACCCGG8300.027.9556986
ATACTCC9200.027.585364
ATACCCG8750.027.3466635
CCATACT9350.027.1428152
ACTCCCC9100.027.0916826
AGGTCGC6100.026.1511826
ACCTATA8650.025.9862771
TATAGGT6450.025.8563143
CCTATAA12750.025.5917971
CATACTC10550.025.4300823