FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677956
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC16980.25045873183510436No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA14810.21845075491624824No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA13710.2022255131601461No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT13610.2007504911823186No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT12540.1849677560195647No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC12040.17759264613042733No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC11950.17626512635038263No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT10520.1551723120674498No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG9980.14720719338718147No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG9980.14720719338718147No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT9290.13702954174017193No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT8910.13142445822442753No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT8070.11903427361067681No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA7840.11564172306167363No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG7370.10870911976588452No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC7330.10811911097475353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCC5200.043.2148448
TCGCGCG6250.042.913749
GTCGCGC6500.041.2632068
CTCCCCC5850.039.6523327
TATAGGT6950.039.6345023
ACCATAC6050.038.3415071
GGTCGCG7150.037.5120057
TGTACGA1209.568881E-636.244712
CCATACT6550.035.4146772
ATACTCC7050.034.9594354
CCCCCCG6650.034.8821269
AGGTCGC7700.034.8325776
CTTCCGA6300.034.5187721
TTAGGAC4050.034.007383
CCGATCT6350.033.10544
TACGGGG900.00363000732.2175184
ACTCCCC7650.032.2175186
CATACTC8050.031.517143
TAAGGTA4850.031.387175
CAGGACT1851.6444028E-731.3467734
Produced by FastQC (version 0.11.2)