FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5c11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5c11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513975
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7920.15409309791332262No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5590.10876015370397393No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5450.10603628581156671No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5220.10156135998832627No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGG657.32568E-444.624732
CTTCCGA18500.034.885681
TTCCGAT19100.033.4101372
CCGATCT19750.032.3105624
CTAACAC1352.1371261E-532.228973
CTGTGTA1153.159703E-431.5222059
TCCGATC22600.028.2363
TATAGTG1555.483822E-528.0731245
TCTAGAC1555.4874603E-528.0703932
TATAGAG1606.807553E-527.1958395
AGTAGAC2156.1036735E-726.9823913
TAGACTG1358.062879E-426.8600865
TCTAACA1100.00965743226.3691562
GTCATAT2301.0993608E-625.2226721
TACCCTC1450.001223442325.007675
CCCTTAT1751.252782E-424.8623491
TAGACGT2101.550641E-524.1717284
GTGTACG1500.001493158424.1670259
AGTAGGA3652.1827873E-1023.8406092
ACAGTGC3352.1027518E-923.8063228
Produced by FastQC (version 0.11.2)