FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5c10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5c10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442583
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11200.2530598780341766No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8390.1895689622059591No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC8390.1895689622059591No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8170.18459814317314494No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA7950.17962732414033075No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT7610.1719451492714361No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC6200.14008671819749063No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT6120.13827914764010366No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6050.13669752340239005No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT5200.11749208623015343No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA5100.11523262303341972No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4830.10913207240223867No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4810.10868017976289193No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA4650.10506503864811798No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4600.10393530704975112No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT4530.10235368281203752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT6100.048.7507824
TTCCGAT6100.048.7507822
CTTCCGA6250.047.5861431
CGTAGGG657.316382E-444.6348652
CGATCTG4450.044.0079735
TCGCGCG2900.042.4945349
GTCGCGC2900.039.9948588
TCCCCCC3500.039.3520858
AGGTCGC3350.038.9722336
TATAGGT2850.038.1745533
GGTCGCG3050.038.0278977
CCATACT4050.037.6090052
TAGGACA1951.6916601E-1037.195724
CCCCCCG3900.037.174719
CTCCCCC3850.035.774627
GTATAAG850.002732966634.1364021
TTAGTAC850.00273448234.132543
ATACTCC5250.033.1573264
TACAGTA1352.1414033E-532.218087
ACTCCCC4300.032.048876
Produced by FastQC (version 0.11.2)