FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403965
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG12230.3027490005322243No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG10360.25645786144839283No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8880.2198210240986224No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC8280.20496825220006684No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA5630.1393685096481131No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5490.13590286287178344No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC5270.13045684650897973No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG5130.1269911997326501No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4870.12055499857660935No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG4590.1136237050239501No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT4480.11090069684254825No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC4340.10743505006621862No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4290.10619731907467231No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT4140.10248412610003342No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC4140.10248412610003342No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4140.10248412610003342No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGT400.006044832554.3828474
CTTCCGA11200.048.5621261
TTCCGAT11500.048.5504842
GTATTAG604.9350265E-448.3462941
CGATCTA2800.046.6138655
TATACTG803.7274967E-545.319045
GATCTAG1002.7273672E-643.5062756
TATTAGC700.001055143141.4345472
CAGTATA700.001055143141.4345474
CCGATCT13450.040.4333464
TCCGATC13850.040.3126753
TACGAGT2550.039.8096664
GTCCTAA1854.1582098E-935.2797281
TCTTAAG1458.671559E-735.005052
ACGAGTC2900.035.005055
AGACAGT1051.8501951E-434.5287936
ACACATA1905.4078555E-934.342808145
GTATACG850.0027364634.1267931
TTATACT850.002738122134.122574
TAACACT850.002738122134.122574
Produced by FastQC (version 0.11.2)