FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068881
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC25610.2395963629253397No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA20440.19122802257688182No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC18300.17120708479241376No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA18190.17017797116797848No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT16590.15520904572164723No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT16190.1514668143600644No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC15600.14594702310172974No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG15050.1408014549795534No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT15040.14070789919551382No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT12970.12134185189932274No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT12380.1158220606409881No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG12100.11320249868788013No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA11390.10656003802107063No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT10800.10104024676273599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA10550.050.8680271
TTCCGAT12550.043.3353732
CCGATCT13150.041.3580974
GGGCGAC3450.033.6204877
TCCCCCC7050.031.8768718
CCCCCCG7550.028.8056329
CTCCCCC8150.027.5744727
ATGTAGG5350.027.1107731
TACGAGT11000.027.0281434
CTAACGT7300.026.8204423
TGTAGGC4600.026.798852
CGATCTG8500.025.5933635
TACTCCC11150.025.3638255
CATACTC9500.025.1892573
CGAGTCG10700.025.0751176
ACTCCCC9850.025.0303946
TCGCGCG7600.024.8006389
TAAGGTG10850.024.7284565
GTACTAC3851.6370905E-1124.4877171
ATACTCC9200.024.4342444
Produced by FastQC (version 0.11.2)