FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289708
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16390.5657420575199856No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.434575503610532No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.34828171814378617No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.2519778535628978No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG451.1889865E-464.482133
TAGACGT1553.438072E-837.441244
CTCTAGG800.002028400336.2712021
TTCCGAT15150.035.912082
CTTCCGA15500.035.5691761
GTGTTAA1655.960828E-835.1720731
TACTGAG1051.8497507E-434.526117
TTGCCTA850.002737890534.119923145
CCGATCT16200.033.5844464
TCCGATC16400.033.174883
ATATGGC900.003613646432.2410666
GATCTTT6400.031.73736
TGTAGAC1153.1457888E-431.5401762
ACCCAAT1153.1553637E-431.523847
CGATCTT7700.031.08965
AATACAG1652.3678822E-630.7755645
TCCCTAC950.00470832430.544171
ACTTACT950.00470832430.544174
ATACAAG950.00470832430.544173
AGGACAG1204.0371442E-430.2260025
Produced by FastQC (version 0.11.2)