FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276342
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14240.515303500734597No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12100.43786322745004375No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.3206172062154866No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.26380354777775367No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.10277120379819209No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC754.838512E-967.665232
CTAGACG1004.674075E-850.7673033
TGTAGGG450.009611960548.358575
CTATAGA907.453364E-540.291514
TCTATGC750.001481680338.6658482
TACACTG1209.504714E-636.268935
ATTGGAC1209.516529E-636.262363
TATGTGT1001.38606E-436.262364
CTTCCGA15800.035.790381
TTCCGAT15750.035.443692
TCCGATC16150.034.578353
GATCTTT7350.032.5680166
GACAGCT1352.1342916E-532.2273757
CCGATCT17600.031.7295654
TTCTAGA1601.870927E-631.7180751
TATATAG1153.146569E-431.5381985
GTGTACA950.004721925630.5256671
CGATCTT9350.029.4806255
TACAAAC1255.1340467E-429.0098883
TCCTATA1000.006067579628.9993862
Produced by FastQC (version 0.11.2)