FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276302
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15780.5711142156046645No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15160.5486750005428842No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8990.32536861839581327No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.23814521791373208No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.11943453178044312No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC451.1894497E-464.4762
CTAGACG651.0860667E-555.816753
GTACTAC1004.661888E-850.7840421
TAGACGT1457.2759576E-1250.0426034
TCTAACC700.001050897841.4638753
GTCTACG907.4388925E-540.3047941
GTACTAA750.001476650738.6926041
GATCTTT7550.036.5211566
CAGACGT1406.5427594E-736.2808884
AGGACTG1301.6408698E-533.490055
GTTATAA900.003611838232.2438351
TGTAGAC900.00361504432.2382
GCCTACA1153.143829E-431.5428831
AATCGGA1153.143829E-431.5428831
TAAGCAA950.004701801630.5523265
TATAAGC950.004701801630.5523263
TTCTAGA950.00470597130.5467951
GTTAATG1204.034632E-430.2285981
CGATCTT10500.029.7157765
ATAGGTG1000.006041750329.024714
Produced by FastQC (version 0.11.2)