FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5b12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5b12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588395
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCTGTACCGATCAATAGTGCGATGTGGGGACGGAGAAGGTTAGCTCAG19000.32291232930259434No Hit
GTACAGGAATATTCACCTGTTTCCCATCAGCTACGCATCTCTGCCTCGCC12050.20479439832085589No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG8030.13647294759472803No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC7230.12287663899251354No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG7160.12168696198981976No Hit
ATACATAGGTGTGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAGCT7110.12083719270218136No Hit
TCATTACTCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT6940.11794797712421078No Hit
GAATACATAGGTGTGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAG6920.11760806940915541No Hit
ATTCTAAGGCGCTTGAGAGAACTCTGGAGAAGGAACTCGGCAAATTGACA6690.11369913068601874No Hit
CCCTATACGTCGACTTTCGTCTTTGCAGAGTGCTGTGTTTTTAATAAACA6240.1060512070972731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGA7100.048.02062
GGAATAT6850.047.6511156
AGGAATA6800.046.9347885
TGTACCG5650.044.9335485
TACCGAT6100.043.970827
GTACCGA5800.041.270256
ACAGGAA7850.040.656893
TCCTGTA6500.040.176972
CTGTACC6850.039.1798064
GTATATC750.001478080938.6922261
ACCGATC7350.037.4790848
TATAGGT5050.037.345053
TAGAGTT1209.5250525E-636.267794
TACACAA1854.1727617E-935.272587145
GTACAGG10850.034.7695121
TCCGATC15550.034.5185433
CCGATCT15350.034.495754
CAGGAAT9550.033.4195374
CCCTACA1551.4570796E-632.760792
TAGACGT2257.876224E-1032.2380374
Produced by FastQC (version 0.11.2)