FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5b10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5b10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249476
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGT100.0070862006145.010222
ACTAGTT300.001933492672.505113
TAGGACT300.001933492672.505114
CATATAG502.0066071E-458.0040932
CTAGGCA400.006043897554.3788343
ACACTCA701.6915796E-551.7893646
CATATAC450.00962803848.3367421
TTGTACC604.9361814E-448.3367424
GTCCATA450.00962803848.3367421
GTATATA657.32341E-444.618531
CACTAAG750.001480736738.6693951
ATGTACG800.00203298736.2525561
TGTACCA1001.3878813E-436.2525565
TATGCTG850.002737112834.1200525
TTATACC850.002737112834.120052145
GGCATTA850.002739805334.1132167
GTCTTAT900.00362180132.224495145
TACACAG900.00362180132.2244955
TCCTGTA950.004718938430.528472
ATACTGC950.004718938430.528476
Produced by FastQC (version 0.11.2)