FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552595
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT19250.046.7104842
TCCGATC19900.045.5491563
CTTCCGA20400.043.3663671
CCGATCT21150.042.1714134
TCCTAGG1209.54323E-636.257132
CCTATAG800.00203386336.257131
TATACAG3050.030.9105135
CGATCTG11400.029.8989375
TACTAGA2202.2560926E-829.6649252
TATAGGA1000.006066304629.0057032
CGATCTA5200.027.8926225
GATCTTT3950.027.539556
TAGGAGC1855.801083E-627.4378264
ACCATAC3306.184564E-1126.368821
CATACTC1958.731888E-626.0307583
CTAGGGT1409.977455E-425.897953
ATCTAAG3408.913048E-1125.5932672
TGCTCTT13950.025.4709572
CTATTAT3151.029548E-925.322441
CTAGACG1450.00122434425.0049154
Produced by FastQC (version 0.11.2)