FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865125
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA12380.14310070799017485No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC12250.14159803496604537No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA9740.11258488657708424No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9390.10853922843519723No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9100.10518711168906228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA12400.043.8573951
CCGATCT13150.040.2531974
TTCCGAT13850.039.265832
GGTCGCG5800.032.497377
TATAGGT6350.029.68953
GTCGCGC6450.028.0985018
CGATCTG8100.027.7510855
TCGCGCG6600.027.4598989
TAAGGTG7150.027.3817375
GTGTATA2756.7520887E-926.36761
CTATACT3306.184564E-1126.3675984
AGGTCGC6900.026.2720646
CATAAGG8400.025.896753
AGGTGCC7450.025.2999677
CCTACAT3451.07320375E-1025.2211823
ATAAGGT8100.025.0654964
TCCGATC21700.025.061373
TCCCCCC4950.024.8969758
GTCTACG7900.024.7822041
TCTATAC5000.024.6537063
Produced by FastQC (version 0.11.2)