FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800373
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA15490.193534764416091No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15210.19003639553058388No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC12760.15942566778239645No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA11920.14893056112587508No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11490.1435580660517034No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT10140.1266909303537226No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT9990.12481680416505804No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT9890.12356738670594836No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8910.11132309560667338No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT8770.10957391116391982No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8670.10832449370481013No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA8570.10707507624570045No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT8210.10257717339290555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT9800.042.9166874
CTTCCGA9900.041.018251
TTCCGAT10700.039.9845772
TCCCCCC4900.038.465018
TCGCGCG5200.033.457739
GTCGCGC5200.032.063668
TATAGGT5250.031.7682153
ACCATAC5950.031.6869681
CTCCCCC6000.031.4130947
CCCCCCG6000.031.4130949
CCATACT6250.031.3262232
TATACAG3050.030.907785
AGGTCGC5450.030.602416
ATACTCC6450.030.3548684
GGTCGCG5650.029.5099187
CATACTC7650.026.5412183
GGGCGAC2859.900759E-925.4357017
ACCTATA6350.025.12311
GCCTAGA1450.001224809825.0049671
CTATAAG10900.024.614982
Produced by FastQC (version 0.11.2)