FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822788
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC9900.12032261044157182No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA9540.11594724278915103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG6350.036.539465
CCGATCT10800.034.2398834
GTCGCGC4450.034.2130978
TCGCGCG4500.033.832959
GGTCGCG4400.032.954177
CTTCCGA11500.032.7862241
TTCCGAT11700.031.6060472
AGGTCGC5100.028.4345056
TAATATA3650.027.8112834
GTATATA5550.027.4354551
TATAGGT6100.026.1504233
GTATAAA5000.026.1028771
GTATTAT2808.181814E-925.895711
CCTATAA8400.025.895711
TATAAGC9950.025.5053253
CTATAAG9800.025.1558342
CTATATA2951.425542E-824.578982
TATAAAG6350.023.979022
ACCTATA4600.023.6439111
CTATACG1851.8289156E-423.5161044
Produced by FastQC (version 0.11.2)