FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p5a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p5a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790956
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA14030.17738028411188486No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC13660.17270240063922646No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA11570.14627868048285872No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC11070.13995721633061764No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9900.1251649902143735No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT9130.11542993541992222No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8430.1065798856067847No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT8340.10544202205938132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA10850.043.444261
CCGATCT11000.042.8518374
TTCCGAT11250.041.2497522
TCGCGCG4450.034.215729
GTCGCGC4600.033.099998
TCCCCCC4400.032.95678
AGGTCGC5150.030.9826956
CCCCCCG4900.029.5937739
GGTCGCG5350.028.4598057
TATACAG2054.028352E-728.29995
ATACTCC6350.027.4085674
TTGCGAC3453.6379788E-1227.3258114
CTCCCCC5850.027.2667397
CGATCTA4600.026.8003145
GTATAGA3607.2759576E-1226.1872351
TGCGACC3357.4578566E-1125.9767765
ACTGACC2259.098276E-725.7794658
AGGTGCC7350.025.6479367
TATAGGT5400.025.5157683
CCTATAA11400.025.4450861
Produced by FastQC (version 0.11.2)